Mastering Biotechnology: From Genes to Applications

What you will learn:

First Genetic Engineering Experiment
DNA Transformation Techniques
Polymerase Chain Reaction (PCR)
DNA Microarray Technology
Reverse Transcription PCR (RT-PCR)

Description

Unlock the world of biotechnology with this in-depth online course!

Dive into the fascinating history and cutting-edge applications of biotechnology. We'll explore fundamental molecular genetics principles, delving into the intricacies of gene expression, DNA replication, and genetic variation. This course goes beyond theory; you'll master essential lab techniques like Polymerase Chain Reaction (PCR), DNA sequencing (both manual and automated), and DNA fingerprinting.

Learn how these techniques are used in diverse fields, including disease diagnosis, forensic science, genetic engineering, and agricultural biotechnology. We'll examine the power of recombinant DNA technology, explore the applications of microchip arrays, and unravel the potential of gene therapy.

Practical applications covered include:

DNA amplification for cloning and sequencing using PCR
Gene mapping and identification of disease-related genes
Pathogen detection and identification
Forensic DNA analysis (including paternity testing)
Applications in agriculture and food safety (GMO detection)
Understanding and using different DNA sequencing techniques.
Principles and applications of gene therapy.
Working with transgenic animals and their importance in research.

This course provides a strong foundation in both the theoretical and practical aspects of modern biotechnology, equipping you with valuable skills for a wide range of careers.

Detailed explanations, practical examples, and real-world case studies ensure a comprehensive learning experience. Enroll now and embark on your biotechnology journey!

Curriculum

Introduction to Biotechnology

This introductory section sets the stage, covering the historical evolution of biotechnology, its prevalent applications in modern life, and a chronological overview of key milestones. You'll also gain a foundational understanding of core biotechnology lab methods, providing a solid base for more advanced topics. Lectures cover: Biotechnology today and yesterday; daily life applications; a detailed timeline; a general introduction to lab methods; and a comprehensive overview of common laboratory techniques.

Recombinant DNA Technology

This section delves into the powerful techniques of recombinant DNA technology. You will learn the step-by-step process of cloning, the function and application of restriction enzymes in genetic engineering, and an in-depth understanding of gene vectors and cloning vectors. We will also cover genome libraries, analysis of restriction sites, and the fundamentals of PCR. Lectures cover: Cloning steps; recombinant DNA concepts; restriction enzymes; their applications in genetic engineering; gene vectors; cloning vectors (lect 2); genome libraries; analysis of restriction sites; and a detailed explanation of the Polymerase Chain Reaction (PCR) (2 lectures).

Microarray Technology

Explore the world of microarrays and gene chip technology. This section explains the principles and applications of this high-throughput technology, specifically focusing on its role in analyzing gene expression and its use in various applications. Lectures will cover: An introduction to microarrays; microchip arrays in detail; gene chip technology; microarray technology explained; and a case study on microarrays for melanoma and breast cancer research.

DNA Sequencing Techniques

Master the art of DNA sequencing, a cornerstone of modern biology. This section covers both chemical/enzymatic methods and the famous Sanger method, supplemented by explanations of gel electrophoresis. The different methods of DNA sequencing are compared and contrasted. Lectures include: Chemical and enzymatic DNA sequencing methods; step-by-step DNA sequencing; Sanger sequencing in detail; gel electrophoresis principles; DNA sequencing methods overview; and a comprehensive overview of DNA sequencing techniques.

Gene Therapy and Applications

This section explores the exciting field of gene therapy, covering its underlying principles, the types of diseases it targets, and the different vectors used in gene therapy. We'll also discuss genetic engineering techniques related to antibody development. Lectures cover: What is gene therapy?; diseases successfully treated with gene therapy; vectors used in gene therapy; and antibody genetic engineering.

Transgenic Animal Technology

This final section provides an introduction to the use of transgenic animals in biotechnology research. Lectures cover the fundamentals of creating and using transgenic animals.

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